Biological reference materials for extracellular vesicle studies

Development of Multi-Purpose Biological Reference Materials

Protein Complexes in Urine Interfere with Extracellular Vesicle Biomarker Studies

Urine exosomes (extracellular vesicles; EVs) contain (micro)RNA (miRNA) and protein biomarkers that are useful for the non-invasive diagnosis of various urological diseases. However, the urinary Tamm-Horsfall protein (THP) complex, which forms at reduced temperatures, may affect EV isolation and may also lead to contamination by other molecules including microRNAs (miRNAs). Therefore, we compar...

Updating the MISEV minimal requirements for extracellular vesicle studies: building bridges to reproducibility

An editorial describing “minimal experimental requirements for definition of extracellular vesicles (EVs)”, or more simply “minimal information for studies of EVs (MISEV)” was published in the Journal of Extracellular Vesicles in late 2014 [1]. Similar to guidelines in other scientific fields [2–4], “MISEV2014”, as we will call it here, provided recommendations on experimental methods and minim...

Biological materials: Fishing for compliance.

692 nature materials | VOL 7 | SEPTEMBER 2008 | www.nature.com/naturematerials constructed chiral anionic layers based on the bridged metal centres. The synthesis involves a subtle enantioselective interaction between a solution of enantiopure cations and a racemic mixture (equal amounts of the two enantiomers) of the metallic components. The reaction leads to the formation of the desired enant...

Performance criteria for reference measurement procedures and reference materials.

The concept of metrological traceability of measurement results to property values assigned to measurement standards of higher metrological order or to the International System of Units (SI) through sequential calibrations, using reference materials and reference measurement procedures, plays a key role in ensuring that end user measurement procedures perform at an acceptable level in the clini...